In
Vitro Study of Human Tenon's Capsule Fibroblasts from Various Types of Glaucoma
A pilot study: comparative with pediatric and cataract patients
Ngamkae
Roengvoravate, M.D.*
Edward N. Burney, M.D.**
* Department of
Ophthalmology, Siriraj Hospital, Bangkok, Thailand.
** Department of Ophthalmology, Case Western Reserve University, Cleveland,
Ohio, USA.
ABSTRACT : The proliferation of fibroblasts and production of
collagen contribute to the failure of glaucoma filtration surgery. The rate of filtration
failure varies according to the type of glaucoma. Therefore, the authors studied the
proliferation and protein synthesis on cultured human Tenon's capsule fibroblasts (CHTCF)
harvested from various types of glaucoma.
IffCF from most
of the glaucoma patients had the same proliferative rate as that from control
cataract patients, but they had a significantly higher protein synthesis,
especially in cell-secreted protein. The high level of protein synthesis
was similar to that from ~CF from pediatrics. These results suggest that
there may be external factors which enhance metabolic activity of fibroblasts
in glaucoma patients.
In addition, the clinical
implications of this study may possibly support that chemotherapeutic
agents adjunctive to trabeculectomy should stress in down-regulating fibroblast
function more than suppres sing of proliferation alone.
Thai J Ophthalmol 1994
: July - December 8(2): 127-134.
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